How to Choose the Appropriate Posterior Slope Angle Can Lead to Good Knee Joint Function Recovery in Total Knee Arthroplasty?

Objective: In this study, we aim to examine the effects of osteotomy under varying posterior slope angles on knee joint function recovery following knee arthroplasty. Methods: We conducted a retrospective analysis from September 2015 to September 2018 on 240 patients who underwent knee arthroplasty three years previously. The study participants were categorized based on changes in the angle of the posterior slope before and after surgery: Group 1, > 5°; Group 2, 3°-5°; Group 3, 0°-3°; Group 4, -3°-0°; Group 5, < -3°. All participants were affected with knee osteoarthritis. The Knee Society Clinical Rating System (KSS) knee function score, Western Ontario and McMaster Universities Arthritis Index (WOMAC) knee function score, Visual Analogue Scale (VAS) pain score, and postoperative complications were measured 3 years after surgery. Results: The level of pain experienced by the patients decreased significantly than before, with pain scores ranging from 1.0-3.0, and there was a statistical difference between groups (H = 93.400, P < 0.001). The KSS score increased, with group 5 having the lowest median score of 78.0 and group 2 having the highest median score of 97.0, and there was a statistical difference between groups (H = 164.460, P < 0.001). The WOMAC score was reduced, with the median score being 24.0, 11.0, 14.0, 20.0, and 26.0, in the five groups, respectively. Group 5 had the highest score, while Group 2 had the lowest score, and there was a statistically significant difference between groups (H = 164.223, P < 0.001). No symptoms such as periprosthetic femoral fracture, prosthetic loosening, or pad wear were detected in patients postoperatively. Conclusion: Osteotomy at various posterior slope angles in total knee arthroplasty impacts postoperative knee function rehabilitation. An excessive increase or decrease in angle can have an impact on the postoperative recovery of knee function.

An investigation into whether changes in the posterior tibial slope affect the outcome of cruciate-retaining total knee arthroplasty by affecting tibiofemoral articular contact kinematics.

Aims: The outcomes of total knee arthroplasty (TKA) are affected by many factors. This study aims to evaluate whether changes in the posterior tibial slope (PTS) affect patients' outcomes after cruciate-retaining TKA by affecting tibiofemoral articular contact kinematics. It was hypothesized that changes in PTS affect the outcomes of PCR TKA by affecting tibiofemoral articular contact kinematics. Methods: A total of 60 knees (30 patients) that underwent posterior cruciate-retaining TKA (with the same size prosthesis) for medial osteoarthritis were assessed preoperatively and one year postoperatively. Before and after TKA, changes in the PTS, as seen on lateral radiographs, were noted. The knees were placed in groups according to these PTS changes (preoperative value - postoperative value): group 1 >3° change and group 2 ≤3° change. Knee kinematics were observed under mid-flexion weight-bearing conditions and were compared between the two groups using the two-dimensional/three-dimensional registration technique. Pain was measured using the visual analog scale, and knee function was assessed using the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) and the Knee Society Score (KSS). Results: Group 2 experienced paradoxical anterior motion of the medial femoral condyle postoperatively, but group 1 did not. A comparison of the results of the TKA between the two groups showed a significant difference in pain using the visual analog scale, and knee function of the KSS and the WOMAC (P < 0.05). The postoperative results were better in group 1 than in group 2. Conclusions: These results suggest that achieving a greater change in the PTS improves outcomes in patients undergoing posterior cruciate-retaining TKA because it reduces the paradoxical motion of the medial femoral condyle.

Different biosynthesis patterns among flavonoid 3-glycosides with distinct effects on accumulation of other flavonoid metabolites in pears (Pyrus bretschneideri Rehd.).

Flavonoid biosynthesis profile was clarified by fruit bagging and re-exposure treatments in the green Chinese pear 'Zaosu' (Pyrus bretschneideri Rehd.) and its red mutant 'Red Zaosu'. Two distinct biosynthesis patterns of flavonoid 3-glycosides were found in 'Zaosu' pear. By comparison with 'Red Zaosu', the biosynthesis of flavonoid 3-galactosides and flavonoid 3-arabinosides were inhibited by bagging and these compounds only re-accumulated to a small degree in the fruit peel of 'Zaosu' after the bags were removed. In contrast, the biosynthesis of flavonoid 3-gluctosides and flavonoid 3-rutinosides was reduced by bagging and then increased when the fruits were re-exposed to sunlight. A combination of correlation, multicollinearity test and partial-correlation analyses among major flavonoid metabolites indicated that biosynthesis of each phenolic compound was independent in 'Zaosu' pear, except for the positive correlation between flavonoid 3-rutincosides and flavanols. In contrast with the green pear cultivar, almost all phenolic compounds in the red mutant had similar biosynthesis patterns except for arbutin. However, only the biosynthesis of flavonoid 3-galactosides was relatively independent and strongly affected the synthesis of the other phenolic compounds. Therefore, we propose a hypothesis that the strong accumulation of flavonoid 3-galactosides stimulated the biosynthesis of other flavonoid compounds in the red mutant and, therefore, caused systemic variation of flavonoid biosynthesis profiles between 'Zaosu' and its red mutant. This hypothesis had been further demonstrated by the enzyme activity of UFGT, and transcript levels of flavonoid biosynthetic genes and been well tested by a stepwise linear regression forecasting model. The gene that encodes flavonoid 3-galacosyltransferase was also identified and isolated from the pear genome.

[The expression level of FtsZ protein during the dedifferentiation and redifferentiation of explants of Nicotiana tobacum].

The explants cells of Nicotiana Tobacum undergo the process of dedifferentiation and redifferentiation under the treatment of phytohormone with different contentrations. By monitoring the change of plastids and the expression of FtsZ protein, which functions in the progress of chloroplast division, we show that chloroplasts turn into amyloid and hypostasis when explants dedifferent and at the same time the expression of FtsZ proteins decreases. When explants redifferent and bud, hypostasis turn back into the chloroplast and the expression of FtsZ protein restores. The concurrence of fluctuant expression of FtsZ protein and the progress of bud differentiation and formation of chloroplast play essential roles in the growth of green plant.